In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
Artesunate is part of the artemisinin group of agents with an IC50 of < 5 μM for small cell lung carcinoma cell line H69. It is a potential inhibitor of STAT-3 and exhibits selective cytotoxicity of cancer cells over normal cells in vitro; this compound is a potent inhibitor of EXP1.
Targets
EXP1(a membrane glutathione S-transferase)
STAT3
In vitro
ART could alter the biomechanical properties of the glioma cells to inhibit cell proliferation, migration and invasion .
This compound significantly suppresses the cell proliferation, induces apoptosis and promoted cell cycle arrest at G0/G1 phase in SKM-1 cells. The mechanisms of its anti-MDS are associated with the increase of intracellular calcium ion concentration and ROS levels. In addition, the pro-apoptotic activity of this chemical may be involved in the regulation of BCL-2/BAX ratio and the expressions of P-bad and survivin . Its treatment demethylates CDH1, which, in turn recovers the E-cadherin activation in SKM-1 cells .
In Vivo
Artesunate is a medication used to treat malaria. This compound can induce radiosensitivity of HeLa cells in vivo using a Xenograft model of nude mice . It had a relatively high immunosuppressive activity with low toxicity, and could inhibit T lymphocyte proliferation induced by mitogen and alloantigen .
Growth inhibition assay: The SKM-1 cells (1×105/mL) are firstly seeded in 96-well plates. Artesunate is diluted in 0.1% dimethyl sulfoxide (DMSO) producing 0, 12.5, 25, 50µg/mL concentrations and added to the SKM-1 cells with 100 µl per well. A negative control is treated with 0.1% DMSO. At 0, 24, 48, and 72 hours, same amount of MTT solution is added into each well and cultured for extra 4 hours. MTT treated cells are fixed with 150 µL DMSO for 30 min at room temperature and then determined with Evolution 201 and 220 UV-Vis spectrophotometer system at 540 nm.
Artesunate Regulates Neurite Outgrowth Inhibitor Protein B Receptor to Overcome Resistance to Sorafenib in Hepatocellular Carcinoma Cells
[ Front Pharmacol, 2021, 12:615889]
Artesunate combined with verteporfin inhibits uveal melanoma by regulation of the MALAT1/yes-associated protein signaling pathway
[ Oncol Lett, 2021, 22(2):597]
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
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