APTSTAT3-9R

Catalog No.S8197 Batch:S819701

Technical Data

Formula

C₂₂₃H₃₃₀N₈₀O₅₁

Molecular Weight

4947.51

CAS No.

no CAS

Solubility (25°C)*

In vitro

Water

100 mg/mL (20.21 mM)

DMSO

10 mg/mL (2.02 mM)

Ethanol

Insoluble

In Vivo (Add solvents to the product individually and in order.)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

APTSTAT3-9R is a specific STAT3-binding peptide with addition of a cell-penetrating motif. The treatment of this compound in various types of cancer cells blocks STAT3 phosphorylation and reduces expression of STAT targets.

Targets

STAT3
(In A549 cells)

In vitro

APTSTAT3-9R effectively inhibited phosphorylation of STAT3 but did not affect the level of AKT phosphorylation and it suppresses cell viability and proliferation of cancer cells.

In Vivo

APTSTAT3-9R could effectively suppress tumour growth in the in vivo allograft and xenograft tumour models by inhibiting STAT3 phosphorylation.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell Lines A549 cells Concentrations 0-100 μmol/L Incubation Time 12 h Method Cells are plated in a 96-well plate (5,000 cells/well) and incubated for 12 hours. After incubation the cells are treated with nine-arginine (9R), APTSTAT3-9R, and this compound for 12 hours. After 12 hours incubation, 20 μL MTT (5 mg/mL) is added to each well. After 4 hours, 100 μL dimethyl sulfoxide is added to each well, and absorbance is measured at 570 nm using a Spectra Macplus microplate reader
Animal Study:^{^[1]}	Animal Models Human lung carcinoma xenograft model(by subcutaneous implantation of A549 cancer cells into female BALB/c nude mice) Dosages 8 mg/kg Administration intratumoral injection

Cell Assay:^{^[1]}

Cell Lines
A549 cells
Concentrations
0-100 μmol/L
Incubation Time
12 h
Method
Cells are plated in a 96-well plate (5,000 cells/well) and incubated for 12 hours. After incubation the cells are treated with nine-arginine (9R), APTSTAT3-9R, and this compound for 12 hours. After 12 hours incubation, 20 μL MTT (5 mg/mL) is added to each well. After 4 hours, 100 μL dimethyl sulfoxide is added to each well, and absorbance is measured at 570 nm using a Spectra Macplus microplate reader

Animal Study:^{^[1]}

Animal Models
Human lung carcinoma xenograft model(by subcutaneous implantation of A549 cancer cells into female BALB/c nude mice)
Dosages
8 mg/kg
Administration
intratumoral injection

References

https://pubmed.ncbi.nlm.nih.gov/24576829/

Sellecks APTSTAT3-9R Has Been Cited by 6 Publications

Hepatic GDP-fucose transporter SLC35C1 attenuates cholestatic liver injury and inflammation by inducing CEACAM1 N153 fucosylation [ Hepatology, 2024, 10.1097/HEP.0000000000001003]	PubMed: 38985995
A transistor model for the cystic fibrosis transmembrane conductance regulator [ Biophys Rep (N Y), 2023, 3(2):100108]	PubMed: 37351179
Establishment and characterization of immortalized sweat gland myoepithelial cells [ Sci Rep, 2022, 12(1):7]	PubMed: 34997030
Milk fat globule EGF factor 8 restores mitochondrial function via integrin-medicated activation of the FAK-STAT3 signaling pathway in acute pancreatitis [ Clin Transl Med, 2021, 11(2):e295]	PubMed: 33634976
Curcumin reverses hepatic epithelial mesenchymal transition induced by trichloroethylene by inhibiting IL-6R/STAT3 [ Toxicol Mech Methods, 2021, 1-11]	PubMed: 34233590
A novel human colon signet-ring cell carcinoma organoid line: establishment, characterization and application [ Carcinogenesis, 2020, 41(7):993-1004]	PubMed: 31740922

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.