In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
Benzamide, a derivative of benzoic acid, is an inhibitor of poly(ADP-ribose) polymerase with an IC50 of 3.3 μM.
Targets
PARP (Cell-free assay)
3.3 μM
In vitro
Benzamide, an inhibitor of PARP is protective against excitatory amino acid-induced cell death in primary cultures of neurons derived from neonatal rat brain. In addition, this compound has more recently been shown to partially prevent the loss of dopaminergic cells and the increase in reactive gliosis caused by METH in vitro in fetal rat mesencephalic cells in culture. It prevents transformation in a cell cycle-specific manner, maximal prevention coinciding with early S phase, also characteristic of maximal susceptibility to transformation.
In Vivo
PARP inhibitor benzamide is neuroprotective in C57Bl/6N mice against different types of neurotoxicities and without affecting body temperature. This compound treatment significantly decreases the iNOS expression and number of apoptotic neurons and thereby improves the neuronal survival and memory during GCI. Its administration (160 mg/kg i.p.) does not induce hypothermia and reaches the CNS in 30 min in the concentration range of 0.09-0.64 mM, at which, it shows neuroprotection.
Exposure to carcinogens and benzamide is done 10 hr after the release of the metabolically induced G1/S block and exposure lasts 10 hr, followed by three washings and refeeding with fresh media.
References
https://pubmed.ncbi.nlm.nih.gov/8911677/
https://pubmed.ncbi.nlm.nih.gov/6196785/
https://pubmed.ncbi.nlm.nih.gov/18501976/
https://pubmed.ncbi.nlm.nih.gov/2538435/
Sellecks Benzamide Has Been Cited by 3 Publications
Unravelling the Regions of Mutant F508del-CFTR More Susceptible to the Action of Four Cystic Fibrosis Correctors.
[ Int J Mol Sci, 2019, 20(21)]
RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
