Crocin

Catalog No.S4758 Batch:S475801

Technical Data

Formula

C₄₄H₆₄O₂₄

Molecular Weight

976.96

CAS No.

42553-65-1

Solubility (25°C)*

In vitro

DMSO

100 mg/mL (102.35 mM)

Water

Insoluble

Ethanol

Insoluble

In Vivo (Add solvents to the product individually and in order.)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Crocin (Alpha-Crocin, Gardenia Yellow) is a water-soluble carotenoid pigment of saffron (Crocus sativus L.). It has been used as a spice for flavouring and colouring food preparations, and in Chinese traditional medicine as an anodyne or tranquiliser.
In vitro	Crocin can significantly inhibit the proliferation of MCF-7 cells, and induce their Apoptosis through mitochondrial signalling pathways including the activation of Caspase-8, upregulation of Bax, the disruption of mitochondrial membrane potential (MMP), and the release of cytochrome c. This compound decreases cell viability in DLA (Daltons lymphoma) cells, in a concentration- and time-dependent manner.
In Vivo	Crocin exhibits a variety of pharmacological effects in mice including inhibition of skin tumour growth, improvement of learning behaviour previously impaired by ethanol, anti-hyperlipidaemic effects, therapeutic efficacy for colon adenocarcinomas in rats, anti-atherosclerotic effects and anti-oxidant effects in PC-12 cells by increasing GSH synthesis. For Daltons lymphoma models, The lifespan of ascites tumour bearing mice treated with this compound, are found to be significantly increasing and a significant reduction in solid tumour volume is found in crocin treated animals when compared to that of control animals. This chemical also shows significant impact on haematological parameters, like the haemoglobin count and numbers of lymphocytes. It has shown that this compound exhibits beneficial effects on many organs including the nervous system, the most studied, gastrointestinal, cardiovascular, genital, endocrine, immune systems, etc. At pharmacological doses it does not harm liver and other organs' function.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell Lines MCF-7 cells Concentrations 10, 25, 50 µg/ml Incubation Time 24 h Method MCF-7 cells were treated with 10, 25, 50 µg/ml Crocin for 24 h and washed with PBS buffer, then fixed in ice-cold 70% ethanol for 15 min. A suspension of 100 µL was taken and incubated with 5 µL propidium iodine (20 µg/ml) and 5 µL annexin V-FITC in the dark for 30 min at room temperature. Finally, 400 µL cold PBS was added to each tube and analyzed by flow cytometry.
Animal Study:^{^[1]}	Animal Models Swiss albino mice injected 1 × 10⁶ Daltons lymphoma cells to the peritoneal cavity Dosages 10 ml/kg Administration oral

Cell Assay:^{^[1]}

Cell Lines
MCF-7 cells
Concentrations
10, 25, 50 µg/ml
Incubation Time
24 h
Method
MCF-7 cells were treated with 10, 25, 50 µg/ml Crocin for 24 h and washed with PBS buffer, then fixed in ice-cold 70% ethanol for 15 min. A suspension of 100 µL was taken and incubated with 5 µL propidium iodine (20 µg/ml) and 5 µL annexin V-FITC in the dark for 30 min at room temperature. Finally, 400 µL cold PBS was added to each tube and analyzed by flow cytometry.

Animal Study:^{^[1]}

Animal Models
Swiss albino mice injected 1 × 10⁶ Daltons lymphoma cells to the peritoneal cavity
Dosages
10 ml/kg
Administration
oral

References

https://pubmed.ncbi.nlm.nih.gov/26884946/
https://pubmed.ncbi.nlm.nih.gov/20104983/
https://pubmed.ncbi.nlm.nih.gov/24275090/

Sellecks Crocin Has Been Cited by 2 Publications

Crocin induces ROS-mediated papillary thyroid cancer cell apoptosis by modulating the miR-34a-5p/PTPN4 axis in vitro [ Toxicol Appl Pharmacol, 2022, 437:115892]	PubMed: 35085590
Rapid and Prolonged Antidepressant-like Effect of Crocin Is Associated with GHSR-Mediated Hippocampal Plasticity-related Proteins in Mice Exposed to Prenatal Stress. [ ACS Chem Neurosci, 2020, 15;11(8):1159-1170]	PubMed: 32203651

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.