In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO
40%PEG300
5%Tween80
50%ddH2O
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
2.35mg/ml
(10.03mM)
Taking the 1 mL working solution as an example, add 50 μL of 47 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
Curcumenol, a sesquiterpene isolated from Curcuma zedoaria, is known to possess a variety of health and medicinal values which includes neuroprotection, anti-inflammatory, anti-tumor and hepatoprotective activities. It inhibits NF-κB activation by suppressing the nuclear translocation of the NF-κB p65 subunit and blocking IκBα phosphorylation and degradation.
Targets
NF-κB
CYP3A4
12.6 μM
In vitro
Curcumenol markedly decreases LPS-induced production of nitric oxide (NO), pro-inflammatory cytokines [(IL-6) and (TNF-α)] and pro-inflammatory proteins expression, iNOS and COX-2. This compound has also been shown to exhibit hepatoprotective properties. It can suppress the LPS-induced NF-κB activity by inhibiting the phosphorylation of Akt. Its treatment significantly inhibits LPS-induced phosphorylation of p38 MAPK but not JNK and ERK. The activity of CYP3A4 is strongly inhibited by this chemical with an IC50 value of 12.6 ± 1.3 μM. Kinetic analysis shows it competitively inhibits testosterone 6β-hydroxylation activity (CYP3A4) with Ki of 10.8 μM.
BV-2 cells are seeded into a sterile flat bottom 96-well plate. The cells are then allowed to adhere overnight. The cells are pretreated with various concentrations of this compound for 2 h followed by LPS exposure (0.4 μg/mL) for 12 h to 24 h.
References
https://pubmed.ncbi.nlm.nih.gov/26301513/
https://pubmed.ncbi.nlm.nih.gov/20148399/
Sellecks Curcumenol Has Been Cited by 3 Publications
Curcumenol Mitigates the Inflammation and Ameliorates the Catabolism Status of the Intervertebral Discs In Vivo and In Vitro via Inhibiting the TNFα/NFκB Pathway
[ Front Pharmacol, 2022, 13:905966]
An Antioxidant Sesquiterpene Inhibits Osteoclastogenesis Via Blocking IPMK/TRAF6 and Counteracts OVX-Induced Osteoporosis in Mice
[ J Bone Miner Res, 2021, 10.1002/jbmr.4328]
Curcumenol mitigates chondrocyte inflammation by inhibiting the NF‑κB and MAPK pathways, and ameliorates DMM‑induced OA in mice
[ Int J Mol Med, 2021, 48(4)192]
RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
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