In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO
Corn oil
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
5.000mg/ml
(10.40mM)
Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
An active component isolated from Tripterygium wilfordii Hook F., Demethylzeylasteral (T-96) inhibits UDP-glucuronosyltransferase (UGT) isoforms UGT1A6 and UGT2B7 with immunosuppressive effects.
Targets
UGT1A6 (Cell-free assay)
UGT2B7 (Cell-free assay)
0.6 μM(Ki)
17.3 μM(Ki)
In vitro
Demethylzeylasteral (T-96) shows strong inhibition towards UGT1A6 and UGT2B7, with negligible influence towards UGT1A9. It has an anti-tumour property in melanoma cells, not only inhibiting cell proliferation through cell cycle arrest at S phase, but also inducing cell apoptosis. CDK2 and Cycin E1 decrease in a dose-dependent manner after its treatment. This compound inhibits MCL1, whose overexpression recovers the proliferation ability inhibited by demethylzeylasteral. It also inhibits clonogenicity and tumorigenesis in melanoma cells through downregulating the expression of MCL1. Furthermore, it is found to inhibit Ca2+ currents in mouse spermatogonia and has an antifertility ability.
In Vivo
Demethylzeylasteral (T-96) demonstrates a significant proteinuria reduction both in a time and concentration-dependent manner. It significantly inhibits the activation of NF-kB in the kidneys of MRL/lpr mice. In addition, this compound reduces the secretion of pro-inflammatory mediators such as TNF-α, COX-2 and ICAM-1.
Demethylzeylasteral (T-96) was used to treat melanoma cell lines MV3 and A375 at different concentrations (1, 5, 10 and 20 μM), with dimethyl sulfoxide (DMSO) as control, for 48 h.
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
