Hydroxy Camptothecine

Catalog No.S3898 Batch:S389801

Technical Data

Formula

C₂₀H₁₆N₂O₅

Molecular Weight

364.35

CAS No.

64439-81-2

Solubility (25°C)*

In vitro

DMSO

29 mg/mL (79.59 mM)

In Vivo (Add solvents to the product individually and in order.)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Hydroxy Camptothecine (10-Hydroxycamptothecin, Hydroxycamptothecine) is a cell-permeable powerful DNA topoisomerase I inhibitor. It has selective inhibitory effect on the phosphorylation of histone H1 and H3, but less effect on other histones.

Targets

DNA topoisomerase I

In vitro

10-HCPT has an inhibitory effect on phosphorylation of histone H1 and H3 in murine hepatoma cells and also exhibits a differentiation inducing effect in human HepG2 cells. 10-HCPT inhibits the cell growth, reduces the cell viability and disturbs the cell cycle distribution of human colon cancer cell line Colo 205. It induces cell cycle arrest in the G2/M phase.

In Vivo

10-HCPT can be safely orally administered and, alternatively, a low-dose long-term treatment. Prolonged elimination of HCPT in vivo may have a significant impact on its therapeutic effects. HCPT is metabolised to its carboxylate form and glucuronides. Dose-dependent toxicity is observed with i.v. administration of HCPT.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell Lines Colo 205 cells Concentrations 5, 10, 15 or 20 nM Incubation Time 24-120 h Method Colo 205 cells (5×10⁵) (ATCC:CCL-222) are seeded in 25T flasks overnight and then treated without (control) and with 5, 10, 15 or 20 nM of HCPT, respectively. After treatment for 24-120 h, cells are harvested by trypsin-EDTA and then centrifuged at 1,500 rpm for 5 min at 4˚C. The cell pellet is resuspended in culture medium containing 0.04% trypan blue and the viable cells are enumerated by a hemocytometer.
Animal Study:^{^[1]}	Animal Models BALB/c-nude mice Dosages 1, 2.5, 5, 7.5 mg/kg Administration oral administration

Cell Assay:^{^[1]}

Cell Lines
Colo 205 cells
Concentrations
5, 10, 15 or 20 nM
Incubation Time
24-120 h
Method
Colo 205 cells (5×10⁵) (ATCC:CCL-222) are seeded in 25T flasks overnight and then treated without (control) and with 5, 10, 15 or 20 nM of HCPT, respectively. After treatment for 24-120 h, cells are harvested by trypsin-EDTA and then centrifuged at 1,500 rpm for 5 min at 4˚C. The cell pellet is resuspended in culture medium containing 0.04% trypan blue and the viable cells are enumerated by a hemocytometer.

Animal Study:^{^[1]}

Animal Models
BALB/c-nude mice
Dosages
1, 2.5, 5, 7.5 mg/kg
Administration
oral administration

References

https://pubmed.ncbi.nlm.nih.gov/16596197/
https://pubmed.ncbi.nlm.nih.gov/9488594/

Sellecks Hydroxy Camptothecine Has Been Cited by 2 Publications

Patient-derived rhabdomyosarcoma cells recapitulate the genetic and transcriptomic landscapes of primary tumors [ iScience, 2024, 27(10):110862]	PubMed: 39319271
Decreasing Arl4c expression by inhibition of AKT signal in human lung adenocarcinoma cells. [ Life Sci, 2020, 246:117428]	PubMed: 32057901

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.