In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
iCRT3 is an antagonist of Wnt/-catenin signalling with an IC50 of 8.2 nM in the Wnt responsive STF16-luc reporter assays.
Targets
Wnt/β-catenin (in STF16 assay)
8.2 nM
In vitro
iCRT3 is a small molecule inhibitor of the Wnt pathway which binds to -catenin interfering with its interaction with TCF. This compound significantly reduces the LPS-induced Wnt/-catenin activity and also inhibits TNF- production and IB degradation in a dose-dependent manner. It does not influence the transcriptional activity of FOP-Flash luciferase reporter, which harbours mutations in the DNA binding sites for TCF (-cat response element), showing specificity of the response. It inhibits cytokine production in LPS-stimulated macrophages.
In Vivo
Intraperitoneal administration of iCRT3 to C57BL/6 mice, subjected to caecal ligation and puncture-induced sepsis, decreases the plasma levels of proinflammatory cytokines and organ injury markers in a dose-dependent manner. The histological integrity of the lungs is improved with this compound treatment, along with reduced lung collagen deposition and apoptosis. In addition, this treatment also decreases the expression of the cytokines, neutrophil chemoattractants, as well as the MPO activity in the lungs of septic mice.
HCT116 cells were cultured in McCoy's 5A medium supplemented with 10% FBS. Cells were treated with specified concentrations of this compound for 1 d and lysed in 50 μL cell lysis buffer at 75℃ for 10 min.
Long non-coding RNA NEAT1 induced by BHLHE40 activates Wnt/β-catenin signaling and potentiates colorectal cancer progression
[ Cell Div, 2024, 19(1):25]
STRAP is a Critical Mediator of APC Mutation-Induced Intestinal Tumorigenesis through a Feed-Forward Mechanism
[ Gastroenterology, 2021, S0016-5085(21)03489-2]
Efficient and robust induction of retinal pigment epithelium cells by tankyrase inhibition regardless of the differentiation propensity of human induced pluripotent stem cells
[ Biochem Biophys Res Commun, 2021, 552:66-72]
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
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