In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
7%DMSO
40%PEG300
5%TWEEN80
48%ddH2O
Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.
7.000mg/ml
(26.39mM)
Taking the 1 mL working solution as an example, add 70 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 480 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
NSC59984 is a p53 pathway activator via induction of mutant p53 protein degradation and p73 activation.
Targets
p53
In vitro
NSC59984 specifically restores p53 pathway signalling and induces p73-dependent cell death in mutant p53-expressing human colorectal cancer cells. This compound also induces cell death in a panel of cancer cell lines bearing different p53 mutations with little or no genotoxicity in normal cells.
In Vivo
In nude mice bearing DLD-1 xenograft tumours, NSC59984 (45 mg/kg, i.p.) significantly represses tumour growth in a p73-dependent manner.
Cells are seeded at 5,000 cells per well on 96-well plates. Cells are mixed with an equal volume of CellTiter-Glo reagents, following the manufacturer's protocol, and bioluminescence imaging is measured using the IVIS imager.
References
https://pubmed.ncbi.nlm.nih.gov/26294215/
Sellecks NSC59984 Has Been Cited by 4 Publications
DRP1 Inhibition Enhances Venetoclax-Induced Mitochondrial Apoptosis in TP53-Mutated Acute Myeloid Leukemia Cells through BAX/BAK Activation
[ Cancers (Basel), 2023, 15(3)745]
Autophagy Inhibition in Trophoblasts Induces Aberrant Shift in CXCR4+ Decidual NK Cell Phenotype Leading to Pregnancy Loss
[ J Clin Med, 2023, 12(23)7491]
RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.
SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
