Oncrasin-1

Catalog No.S0780 Batch:S078001

Technical Data

Formula

C₁₆H₁₂ClNO

Molecular Weight

269.73

CAS No.

75629-57-1

Solubility (25°C)*

In vitro

DMSO

54 mg/mL (200.2 mM)

Ethanol

13 mg/mL (48.19 mM)

Water

Insoluble

In Vivo (Add solvents to the product individually and in order.)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.7mg/ml (10.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.39mg/ml (1.45mM)	Taking the 1 mL working solution as an example, add 50 μL of 7.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Oncrasin-1 (Onc-1) is a potent RNA polymerase II inhibitor with antitumor activity that kills various human lung cancer cells with K-Ras mutations. This compound drives abnormal aggregation of protein kinase C iota (PKC ) in nucleus of sensitive cells but not in resistant cells.

Targets

K-ras

RNA polymerase II

PKCι

In vitro

Oncrasin-1 (NSC-743380) is a selective cytotoxic compound with significant efficacy against lung cancer cells carrying specific KRAS gene mutations. This compound inhibits tumour cell proliferation by inducing cell cycle arrest and promoting apoptosis. Research shows that this chemical has low toxicity to normal cells, indicating good selectivity. Further mechanistic studies suggest that this compound may exert its effects by modulating downstream effector molecules of the RAS signalling pathway. Experiments have demonstrated that the anti-tumour activity of this chemical is positively correlated with its concentration and treatment time. Furthermore, this compound has shown good tolerability and tumour growth inhibitory effects in animal models. Therefore, this chemical holds promise as a potential drug candidate for the treatment of KRAS-mutated lung cancer.

In Vivo

Oncrasin-1 (NSC-743380) is a selective anti-tumour small molecule that acts by inducing apoptosis in cancer cells. This compound was initially discovered during screening for KRAS-mutated lung cancer cells and can effectively inhibit the proliferation of various cancer cell lines. Studies have shown that the mechanism of action of this chemical involves the inhibition of RNA polymerase I, leading to nucleolar stress and cell cycle arrest. Further experiments confirmed that this molecule has little effect on normal cells, demonstrating a good therapeutic window. Currently, this small molecule has become an important tool compound for research into targeted cancer therapy.

Protocol (from reference)

References

https://pubmed.ncbi.nlm.nih.gov/21443218/
https://pubmed.ncbi.nlm.nih.gov/18794128/

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.