PD 169316

Catalog No.S5183 Batch:S518302

Technical Data

Formula

C₂₀H₁₃FN₄O₂

Molecular Weight

360.34

CAS No.

152121-53-4

Solubility (25°C)*

In vitro

DMSO

14 mg/mL (38.85 mM)

Water

Insoluble

Ethanol

Insoluble

In Vivo (Add solvents to the product individually and in order.)

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.700mg/ml (1.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.200mg/ml (0.56mM)	Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

PD 169316 is a potent, selective and cell-permeable p38 MAP kinase inhibitor with an IC50 of 89 nM. PD169316 abrogates signalling initiated by both TGFbeta and Activin A. PD169316 shows antiviral activity against Enterovirus71.

Targets

TGF-β	Activin A	Enterovirus71	p38 MAPK (Cell-free assay)
			89 nM

In vitro

Inhibition of the P38 kinase activity using 0.5 μM PD169316 significantly changes the proliferation rate and extent of apoptosis; the effects of 0.2 μM PD169316 are not significant. The level of P-STAT1 (Tyr701) decreases with 0.5 μM PD169316, which is probably due to P38 inhibition.

In Vivo

PD169316 (intramuscular injection every day for 14 consecutive days) shows antiviral activity in a suckling mouse model.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell Lines KBU cells Concentrations 0.2 μM, 0.5 μM Incubation Time 1 h Method KBU cells are exposed to 12 ppm G. pps alone or with 1 h-pre-exposure to P38 inhibitor PD169316 and analyzed for proliferation (MTT), apoptosis (Ann V) and changes in the level of modifications of proteins involved in the SAPK/JNK and P38 signaling pathways.

References

https://pubmed.ncbi.nlm.nih.gov/34048499/
https://pubmed.ncbi.nlm.nih.gov/28545002/
https://pubmed.ncbi.nlm.nih.gov/14521923/

Sellecks PD 169316 Has Been Cited by 2 Publications

Pregnancy-to-lactation transition remodels mammary adipocytes via p38 MAPK-dependent adipogenesis [ J Dairy Sci, 2025, S0022-0302(25)00833-1]	PubMed: 41130403
Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathway [ PLoS One, 2021, 16(5):e0252541]	PubMed: 34048499

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.