SPHINX31

Catalog No.S8810 Batch:S881001

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Technical Data

Formula

C27H24F3N5O2
Molecular Weight 507.51 CAS No. 1818389-84-2
Solubility (25°C)* In vitro DMSO 25 mg/mL (49.26 mM)
Ethanol 13 mg/mL (25.61 mM)
Water Insoluble
In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 1.250mg/ml (2.46mM) Taking the 1 mL working solution as an example, add 50 μL of 25 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.

 0.200mg/ml (0.39mM) Taking the 1 mL working solution as an example, add 50 μL of 4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description SPHINX31 is a SRPK1 inhibitor with an IC50 of 5.9 nM with high selectivity for SRPK1 over SRPK2 (50-fold) and CLK1 (100-fold).
Targets
SRPK1
(Cell-free assay)
5.9 nM
In vitro

Kinase assays showed that SPHINX31 is a type 1 kinase inhibitor (ATP competitive). This compound treatment results in inhibition of SRSF1 phosphorylation at 300 nM in PC3 prostate cancer cells. Metabolic stability in mouse liver microsomes shows that this chemical had medium clearance with a T1/2 of 95.79 min. Inhibition of SRPK1 using this inhibitor leads to cell cycle arrest and leukemic cell differentiation.
In Vivo

SPHINX31 could penetrate into the eye. This compound exerts a dose dependent inhibition of choroidal neovascularisation in mouse model. It inhibits blood vessel growth and macrophage infiltration. This chemical treatment prolongs survival of immunocompromised mice transplanted with MLL-rearranged AML cells.

Protocol (from reference)

Cell Assay:

[2]
  • Cell Lines

    THP1 cells

  • Concentrations

    3 µM

  • Incubation Time

    24 h

  • Method

    --
Animal Study:

[2]
  • Animal Models

    DBA2J mice

  • Dosages

    0.8 mg/kg

  • Administration

    i.p.

References

  • https://pubmed.ncbi.nlm.nih.gov/28135068/
  • https://pubmed.ncbi.nlm.nih.gov/30568163/

Sellecks SPHINX31 Has Been Cited by 2 Publications

Posttranslational splicing modifications as a key mechanism in cytarabine resistance in acute myeloid leukemia [ Leukemia, 2023, 37(8):1649-1659] PubMed: 37422594
SRPKIN-1 as an inhibitor against hepatitis B virus blocking the viral particle formation and the early step of the viral infection [ Antiviral Res, 2023, 10.1016/j.antiviral.2023.105756] PubMed: 37992764

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.