In Vivo (Add solvents to the product individually and in order.)
Homogeneous suspension
CMC-NA
≥5mg/ml
Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
Biological Activity
Description
Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.
Targets
cysteine protease
In vitro
Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. This compound inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. It efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro.
In Vivo
Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. This compound blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice.
T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester.
References
https://pubmed.ncbi.nlm.nih.gov/4073501/
https://pubmed.ncbi.nlm.nih.gov/2069557/
https://pubmed.ncbi.nlm.nih.gov/11290751/
https://pubmed.ncbi.nlm.nih.gov/16951345/
https://pubmed.ncbi.nlm.nih.gov/20834102/
Sellecks Z-FA-FMK Has Been Cited by 11 Publications
Fluorescence-guided tumor resection with a cathepsin B-activatable, EGFR-targeted probe and a dual-mode surgical exoscope
[ Eur J Med Chem, 2025, 300:118108]
Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy
[ Comput Struct Biotechnol J, 2022, 20:2442-2454]
Targeting multiple cell death pathways extends the shelf life and preserves the function of human and mouse neutrophils for transfusion
[ Sci Transl Med, 2021, 13(604)eabb1069]
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.
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