Name: Daidzein
Brand: Selleck Chemicals
SKU: S1849
Rating: 5 (2 reviews)

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Quality Control

Batch: Purity: 99.99%

99.99

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase Vitamin Carbohydrate Metabolism Mitochondrial Metabolism Drug Metabolite
Other PPAR Inhibitors	T0070907 GW9662 GW6471 WY-14643 (Pirinixic Acid) GSK3787 GW0742 AZ6102 Harmine Astaxanthin Eupatilin

Chemical Information, Storage & Stability

Molecular Weight	254.24	Formula	C₁₅H₁₀O₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	486-66-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1=CC(=CC=C1C2=COC3=C(C2=O)C=CC(=C3)O)O

Solubility

In vitro
Batch:

DMSO : 51 mg/mL (200.59 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.55mg/ml (10.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 51 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.36mg/ml (1.42mM)	Taking the 1 mL working solution as an example, add 50 μL of 7.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	Daidzein inhibits iNOS protein and mRNA expression and also NO production in a dose-dependent manner. This compound also inhibits the activation of the signal transducer and activator of transcription 1 (STAT-1), another important transcription factor for iNOS. This chemical (1 μM and 10 μM) causes a significant elevation of protein content, alkaline phosphatase activity, and DNA content in cells; those increases are about 1.4-, 1.5-, and 2.0-fold, respectively. It (2-50 mM) increases the viability of osteoblasts by about 1.4-fold. This compound (2-100 mM) increases the alkaline phosphatase activity and osteocalcin synthesis of osteoblasts by about 1.4- and 2.0-fold, respectively. It stimulates osteoblast differentiation at various stages (from osteoprogenitors to terminally differentiated osteoblasts). This chemical (1 μM and 10 μM) causes a significant increase of alkaline phosphatase activity, deoxyribonucleic acid (DNA) and calcium contents in bone tissues. The compound-induced increase of calcium content and alkaline phosphatase activity in bone tissues is completely prevented by cycloheximide (1 μM).
In vivo	Daidzein results in reduced body weight, a fact that may be explained by reduced feed consumption in female rats. This compound results in slight, but not significant, decreases in ovarian and uterine weights, and mammary gland size in female rats.
References	[1] https://pubmed.ncbi.nlm.nih.gov/18274639/ [2] https://pubmed.ncbi.nlm.nih.gov/10660113/ [3] https://pubmed.ncbi.nlm.nih.gov/12628484/ [4] https://pubmed.ncbi.nlm.nih.gov/10391128/ [5] https://pubmed.ncbi.nlm.nih.gov/11812927/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05073523	Completed	Healthy	Chalmers University of Technology	September 27 2021	Not Applicable

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Handling Instructions

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Daidzein PPAR activator

Chemical Structure

Molecular Weight: 254.24