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Capsaicin(Vanilloid) TRP Channel Agonist

Name: Capsaicin(Vanilloid)
Brand: Selleck Chemicals
SKU: S1990
Rating: 5 (16 reviews)

Cat.No.: S1990

Capsaicin(Vanilloid) is an active component of chili peppers, which are plants belonging to the genus Capsicum. It is a TRPV1 agonist with an EC50 of 0.29 μM in HEK293 cells. This compound is a mixture of Capsaicin and Dihydrocapsaicin.

Chemical Structure

Molecular Weight: 305.41

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Quality Control

Batch: Purity: 99.86%

99.86

Related Targets	CFTR CRM1 CD markers AChR Calcium Channel Sodium Channel Potassium Channel GABA Receptor ATPase GluR
Other TRP Channel Inhibitors	2-APB (2-Aminoethyl Diphenylborinate) SKF96365 AMG-517 Capsazepine GSK2193874 HC-030031 GSK1016790A EIPA (L593754) ML-SI3 SB705498

Chemical Information, Storage & Stability

Molecular Weight	305.41	Formula	C₁₈H₂₇NO₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	404-86-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

DMSO : 61 mg/mL (199.73 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 61 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.700mg/ml (8.84mM)	Taking the 1 mL working solution as an example, add 50 μL of 54 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.050mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL 61 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	As a TRPV-1 agonist, Capsaicin (Vanilloid) dose-dependently inhibits the proliferation of the HCC cell lines PLC/PRF/7, HuH7 and HepG2, which is not effected by TRPV1 channel blockers. Combining it and sorafenib significantly enhances the suppression on cell proliferation, achieving a high-level synergistic effect and promoting HCC cell apoptosis through the enhancement of p-ERK and reduction of p-STAT3 signaling.
In vivo	Combining capsaicin (vanilloid) and sorafenib enhances inhibition of xenograft tumor growth in nude mice, with no apparent gross toxicity.
References	[1] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5843835/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-08-21)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03952845	NOT_YET_RECRUITING	Tolerance; Capsaicin; Rhinitis	Nova Scotia Health Authority	2025-08-01
NCT07443553	NOT_YET_RECRUITING	Postamputation Pain	Northwestern University	2026-10-31
NCT05997979	RECRUITING	Neuropathic Pain; Chronic Pain	University Hospital, Brest	2024-03-05	PHASE3
NCT03511846	ENROLLING_BY_INVITATION	Migraine; Cluster Headache; Trigeminal Autonomic Cephalgia; Hemicrania Continua; Paroxysmal Hemicrania; SUNCT; Short-Lasting Unilateral Neuralgiform Headache With Conjunctival Injection and Tearing	The University of Texas Health Science Center, Houston	2018-03-21	PHASE1
NCT07660510	NOT_YET_RECRUITING	Neuropathic Pain; Nociplastic Pain	Nantes University Hospital	2026-09-01
NCT05840562	RECRUITING	Chemotherapy-induced Peripheral Neuropathy	Institut Cancerologie de l'Ouest	2023-10-20	PHASE3

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