For research use only. Not for use in humans.

Denosumab (anti-RANK ligand)

Synonyms: AMG-162 Cat.No.A2020 Purity:99.68%

Denosumab (anti-RANK ligand) (AMG-162) is a fully human monoclonal antibody that selectively inhibits primate RANKL. Denosumab inhibits the ability of human RANKL (143–317) to stimulate the formation of osteoclasts derived from murine RAW 264.7 cells with an IC50 value of 1.64 nM.

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4 Citations

17 Nobel Prize winners
have used Selleck products

Shimon Sakaguchi

Nature Communications 2025;16, Article number:1325

David Baker

Dev Cell 2023;58(20):2163-2180.e9.

Michael Houghton

Cell Chem Biol 2020;27(7):780-792.e5

David Julius

Cell 2017;185-198.e16

Charles M. Rice

Cell 2018;172(3):423-438.e25

Quality Control

Batch: Purity: 99.68% Protein concentration: 5mg/ml Endotoxin Level: <1EU/mg

99.68

Specificity

Name	Citation	NF-κB	Others
QNZ (EVP4593)	156	++++	TNF-α
JSH-23	166	+
CBL0137 Hydrochloride	9	++	FACT,p53
SC75741	53	+++
Curcumin	50	✔	HDAC,Nrf2,p300 histone acetylatransferase
Sodium 4-Aminosalicylate	2	✔
Caffeic Acid Phenethyl Ester	55	✔
Sodium salicylate	1	✔
(-)-Parthenolide	27	✔	p53,MDM2 ubiquitination,HDAC1
Andrographolide	11	✔

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1. "+" indicates inhibitory effect. Increased inhibition is marked by a higher "+" designation.
2. "✔" indicates inhibitory effect, but without specific value.

Mechanism of Action

Description	Denosumab (anti-RANK ligand) (AMG-162) is a fully human monoclonal antibody that selectively inhibits primate RANKL. Denosumab inhibits the ability of human RANKL (143–317) to stimulate the formation of osteoclasts derived from murine RAW 264.7 cells with an IC50 value of 1.64 nM.
References	[1] https://pubmed.ncbi.nlm.nih.gov/19016581/ [2] https://pubmed.ncbi.nlm.nih.gov/29435849/

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Product Details

CAS No.	615258-40-7
Molecular Weight	145.16 kDa
Isotype	human IgG1
Source	HEK293
Sterility	0.2 μM filtered
Formulation	PBS Buffer, pH 7.4
Storage	Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles.

Comparison of Clones for

^*Literature analysis of various clone (for this target) products available on the market shows that Sellecks selected clones are more frequently applied. (data until September 2024)

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