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JW55 Wnt/beta-catenin inhibitor

Name: JW55
Brand: Selleck Chemicals
SKU: S6745
Rating: 5 (3 reviews)

Cat.No.S6745

JW55 is a potent and selective inhibitor of the canonical Wnt pathway that functions via inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).

Chemical Structure

Molecular Weight: 434.49

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Quality Control

Batch: S674501 DMSO]87 mg/mL]false]Ethanol]4 mg/mL]false]Water]Insoluble]false Purity: 99.95%

Cited in Nature Medicine for its top-tier quality
COA
SDS
Datasheet

99.95

Related Targets	JAK TGF-beta/Smad ERK GSK-3 ROCK Hedgehog/Smoothened PKA Secretase STAT Casein Kinase
Other Wnt/beta-catenin Inhibitors	IWR-1-endo PRI-724 (Foscenvivint) IWP-2 Tegatrabetan (BC-2059) Isoquercitrin ICG-001 SKL2001 BML-284 Hydrochloride (Wnt agonist 1, AMBMP) PNU-74654 Salinomycin (Procoxacin)

Chemical Information, Storage & Stability

Molecular Weight	434.49	Formula	C₂₅H₂₆N₂O₅	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	664993-53-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	COC1=CC=C(C=C1)C2(CCOCC2)CNC(=O)C3=CC=C(C=C3)NC(=O)C4=CC=CO4

Solubility

In vitro
Batch:

DMSO : 87 mg/mL (200.23 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 4 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Mechanism of Action

Targets/IC₅₀/Ki	Wnt
In vitro	JW55 inhibits the PARP domain of TNKS1/2, leading to the stabilisation of AXIN2 followed by increased degradation of β-catenin. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by this compound with an IC50 value of 470 nmol/L. It decreases auto-PARsylation of TNKS1/2 in vitro with IC50 values of 1.9 μmol/L and 830 nmol/L, respectively. This chemical-mediated inhibition of canonical Wnt signalling results in reduced cell-cycle progression, proliferation, and colony formation in the CRC cell line SW480 in vitro.
In vivo	This compound reduces XWnt8-induced axis duplication in Xenopus embryos and tamoxifen-induced polyposis formation in conditional APC mutant mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/22440753/

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