research use only

Morphothiadin HBV inhibitor

Name: Morphothiadin
Brand: Selleck Chemicals
SKU: E0094
Rating: 5 (1 reviews)

Cat.No.E0094

Morphothiadin (GLS4) is a potent inhibitor on the replication of both wild-type and adefovir-resistant Hepatitis B virus (HBV) with an IC50 of 12 nM. This compound, a nucleoside analog that targets the virus polymerase, can block the HBV replication cycle and HBV-associated chronic liver diseases.

Chemical Structure

Molecular Weight: 509.39

Jump to

Quality Control

Batch: E009401 DMSO]100 mg/mL]false]Ethanol]12 mg/mL]false]Water]Insoluble]false Purity: 99.87%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS
Datasheet

99.87

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV
Other HBV Inhibitors	Bay 41-4109 racemate Bicyclol Besifovir AB-423 JNJ-632 Polygala Fallax Hemsl. Rhizome Extract

Chemical Information, Storage & Stability

Molecular Weight	509.39	Formula	C₂₁H₂₂BrFN₄O₃S	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1092970-12-1	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (196.31 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 12 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (9.82mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.625mg/ml (1.23mM)	Taking the 1 mL working solution as an example, add 50 μL of 12.5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	HBV (in the Southern blotting of analysis of replicative intermediate of HBV) 12 nM
In vitro	In vitro, Morphothiadin (GLS4) is significantly less toxic for primary human hepatocytes, and inhibits virus accumulation in the supernatant of HepAD38 cells and HBV replicative forms in the liver significantly, also strongly inhibits core gene expression.;
In vivo	For nude mice inoculated with HepAD38 cells, treatment with this compound shows strong and sustained suppression of virus DNA to about the same extent both during and after treatment, and reduces the levels of intracellular core antigen in the tumours.
References	[1] https://pubmed.ncbi.nlm.nih.gov/23959305/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):