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Salermide Sirtuin inhibitor

Name: Salermide
Brand: Selleck Chemicals
SKU: S8460
Rating: 5 (3 reviews)

Cat.No.S8460

Salermide is a reverse amide with a strong in vitro inhibitory effect on Sirt1 and Sirt2. Compared with Sirt1, this compound is even more efficient at inhibiting Sirt2.

Chemical Structure

Molecular Weight: 394.47

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Quality Control

Batch: S846001 DMSO]78 mg/mL]false]Ethanol]8 mg/mL]false]Water]Insoluble]false Purity: 99.79%

Cited in Nature Medicine for its top-tier quality
COA
SDS
Datasheet

99.79

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN DNA/RNA Synthesis Topoisomerase PPAR Casein Kinase eIF
Other Sirtuin Inhibitors	SRT 1720 Hydrochloride Selisistat (EX-527) Sirtinol Fisetin 3-TYP AGK2 SRT2104 (GSK2245840) OSS_128167 SirReal2 Thiomyristoyl

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description
CRO cells	Cytotoxicity assay		72 h	Cytotoxicity against human CRO cells after 72 hrs by celltiter-glo luminescent assay, CC50=6.7 μM
CRC 1.1 cells	Cytotoxicity assay		72 h	Cytotoxicity against human CRC 1.1 cells after 72 hrs by celltiter-glo luminescent assay, CC50=9.7 μM
30PT cells	Cytotoxicity assay		72 h	Cytotoxicity against human 30PT cells after 72 hrs by celltiter-glo luminescent assay, CC50=36.5 μM
30P cells	Cytotoxicity assay		72 h	Cytotoxicity against human 30P cells after 72 hrs by celltiter-glo luminescent assay, CC50=41 μM
MCF7 cells	Proliferation assay	30 μM	24-72 h	Antiproliferative activity against human MCF7 cells at 30 uM after 24 to 72 hrs
U937 cells	Function assay	50 μM	45 h	Induction of apoptosis in human U937 cells at 50 uM after 45 hrs by flow cytometry
MOLT4 cells	Proliferation assay	25 μM	24-72 h	Antiproliferative activity against human MOLT4 cells at 25 uM after 24 to 72 hrs by MTT assay
MDA-MB-231 cells	Proliferation assay	25 μM	24-72 h	Antiproliferative activity against human MDA-MB-231 cells at 25 uM after 24 to 72 hrs by MTT assay
RKO cells	Proliferation assay	25 μM	24-72 h	Antiproliferative activity against human RKO cells at 25 uM after 24 to 72 hrs by MTT assay
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	394.47	Formula	C₂₆H₂₂N₂O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1105698-15-4	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(C1=CC=CC=C1)C(=O)NC2=CC(=CC=C2)N=CC3=C(C=CC4=CC=CC=C43)O

Solubility

In vitro
Batch:

DMSO : 78 mg/mL (197.73 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 8 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	SIRT1 SIRT2
In vitro	Salermide prompts tumour-specific cell death in a wide range of human cancer cell lines. The antitumour activity of this compound is primarily because of a massive induction of apoptosis. It induces apoptosis in cancer but not in normal cells. This chemical induces strong apoptosis without any evident effect on the cell cycle in all the cancer cell lines analysed except in non-tumorigenic MRC5 cells. The induction of apoptosis is cell-type-specific and dose-dependent.
In vivo	Salermide is well tolerated by mice at concentrations up to 100 μM. Its feeding does not produce any adverse health effects in mice as monitored by diet consumption, body-weight gain, and postural and behavioural changes.
References	[1] https://pubmed.ncbi.nlm.nih.gov/19060927/

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