Name: Sodium Nitroprusside Dihydrate
Brand: Selleck Chemicals
SKU: S4059
Rating: 5 (3 reviews)

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Quality Control

Batch: S405901 DMSO]60 mg/mL]false]Water]60 mg/mL]false]Ethanol]Insoluble]false Purity: 99.70%

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COA
SDS
Datasheet

99.70

Related Targets	PROTAC Dyes Antineoplastic and Immunosuppressive Antibiotics ADC Cytotoxin Selection Antibiotics for Transfected Cell Antibiotics for Mammalian Cell Culture ADC Linker Molecular Glues Antibiotics for Plant Cell Culture Hydrotropic Agents
Other Others Inhibitors	Furimazine (PBI 3939) Guanidinoacetic acid Blebbistatin Polybrene (Hexadimethrine Bromide) Silibinin (NSC 651520) Palmitic acid Sesamin Ursolic Acid Osthole Carbazochrome sodium sulfonate (AC-17)

Chemical Information, Storage & Stability

Molecular Weight	297.95	Formula	C₅H₄FeN₆Na₂O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	13755-38-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	[C-]#N.[C-]#N.[C-]#N.[C-]#N.[C-]#N.[N-]=O.O.O.[Na+].[Na+].[Fe+4]

Solubility

In vitro
Batch:

DMSO : 60 mg/mL (201.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 60 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 95 %Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.43mg/ml (1.44mM)	Taking the 1 mL working solution as an example, add 50 μL of 8.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3mg/ml (10.07mM)	Taking the 1 mL working solution as an example, add 50 μL of 60 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	guanylate cyclase
In vitro	Sodium Nitroprusside is a potent vasodilator. Sodium nitroprusside has potent vasodilating effects in arterioles and venules. Sodium Nitroprusside breaks down in circulation to release nitric oxide (NO). NO activates guanylate cyclase in vascular smooth muscle and increases intracellular production of cGMP. The end result is vascular smooth muscle relaxation, which allow vessels to dilate. Sodium nitroprusside decreases the proliferation of vascular smooth muscle cells.
In vivo	Sodium nitroprusside (5 mg/kg) significantly reduces the intestinal ischemiareperfusion injury as a nitric oxide donor in rats.
References	[1] http://www.ncbi.nlm.nih.gov/pubmed/?term=8660704 [2] http://www.ncbi.nlm.nih.gov/pubmed/?term=2540223 [3] http://www.ncbi.nlm.nih.gov/pubmed/?term=18568253

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-12-09)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06424756	RECRUITING	Healthy	University of Georgia	2024-07-01	PHASE2
NCT02560805	RECRUITING	Stress Disorders, Post-Traumatic	Emory University	2015-10	PHASE2
NCT05193968	RECRUITING	Hot Flashes; Menopause; Vasomotor System; Labile	Mayo Clinic	2022-02-24
NCT03318094	ACTIVE_NOT_RECRUITING	Insulin Resistance; Healthy; Obesity	Vanderbilt University Medical Center	2017-10-24	PHASE1
NCT02966665	RECRUITING	Chronic Obstructive Pulmonary Disease; Pulmonary Artery Hypertension; Heart Failure; Hypertension	Russell Richardson	2008-09	PHASE1
NCT04986072	TERMINATED	Schizophrenia; Schizoaffective Disorder	Beth Israel Deaconess Medical Center	2022-03-14	PHASE2

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Sodium Nitroprusside Dihydrate

Molecular Weight: 297.95