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VBIT-12 VDAC inhibitor

Name: VBIT-12
Brand: Selleck Chemicals
SKU: S8936
Rating: 5 (7 reviews)

Cat.No.S8936

VBIT-12 is a potent inhibitor of Voltage-dependent anion-selective channel 1 (VDAC1), directly interacting with purified VDAC1, reducing its channel conductance and preventing VDAC1 oligomerisation.

Chemical Structure

Molecular Weight: 417.50

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Quality Control

Batch: S893601 DMSO]83 mg/mL]false]Water]2 mg/mL]false]Ethanol]Insoluble]false Purity: 98.69%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

98.69

Related Targets	CFTR CRM1 CD markers AChR Calcium Channel Sodium Channel Potassium Channel GABA Receptor TRP Channel ATPase
Other VDAC Inhibitors	VBIT-4 NSC15364 DIDS sodium salt

Chemical Information, Storage & Stability

Molecular Weight	417.50	Formula	C₂₅H₂₇N₃O₃	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	2089227-65-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1CN(CCC1(C(=O)NCC(=O)O)NC2=CC=CC=C2)CC3=CC=CC4=CC=CC=C43

Solubility

In vitro
Batch:

DMSO : 83 mg/mL (198.8 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 2 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.150mg/ml (9.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 83 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.030mg/ml (2.47mM)	Taking the 1 mL working solution as an example, add 50 μL of 20.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	VDAC1
In vitro	VBIT-12 directly interacts with purified VDAC1 and reduces its channel conductance. This compound interferes with VDAC1 oligomerisation and thus with the pro-apoptotic action of VDAC1 when the levels of expression are abnormal, but has no effect on cells under physiological conditions.
In vivo	The effect of VBIT-12 on induction of startle habituation and prepulse inhibition (PPI) in animals is examined via novelty suppressed feeding test, sweet preference model of anhedonia and forced swim test (FST) model for despair. This compound interacts with the Voltage-Dependent Anion Channel (VDAC) and can be used for the treatment of central nervous system (CNS) disorders and diseases.
References	[1] https://patents.google.com/patent/US20200000801A1 [2] https://pubmed.ncbi.nlm.nih.gov/29551631/

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