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WIN 55, 212-2 mesylate Cannabinoid Receptor agonist

Name: WIN 55, 212-2 mesylate
Brand: Selleck Chemicals
SKU: S8017
Rating: 5 (1 reviews)

Cat.No.S8017

WIN 55,212-2 Mesylate ((R)-(+)-WIN 55212) is a potent cannabinoid (CB) receptor agonist with Ki of 3.3 nM and 62.3 nM for human recombinant CB2 and CB1, respectively. WIN 55,212-2 Mesylate regulates Glutamate transmission.

Chemical Structure

Molecular Weight: 522.61

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Quality Control

Batch: Purity: 99.37%

99.37

Related Targets	CXCR Hedgehog/Smoothened PKA Adrenergic Receptor AChR 5-HT Receptor Histamine Receptor Dopamine Receptor Ras KRas
Other Cannabinoid Receptor Inhibitors	AM1241 AM251 BML-190 Otenabant (CP-945598) HCl 6-Iodopravadoline (AM630) Org 27569 GW842166X CID16020046 Yangonin (+)-Gallocatechin

Chemical Information, Storage & Stability

Molecular Weight	522.61	Formula	C₂₈H₃₀N₂O₆S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	131543-23-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	(R)-(+)-WIN 55212			Smiles	CC1=C(C2=C3N1C(COC3=CC=C2)CN4CCOCC4)C(=O)C5=CC=CC6=CC=CC=C65.CS(=O)(=O)O

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (191.34 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	CB2 (Cell-free assay) 3.3 nM(Ki) CB1 (Cell-free assay) 62.3 nM(Ki)
In vitro	WIN 55,212-2 increases, in a nanomolar concentration range, extra-cellular glutamate levels in primary cultures of rat cerebral cortex neurons, displaying a bell-shaped concentration-response curve. The aminoalkylindole WIN 55,212-2 is more selective for the CB2 than the CB1 receptor, with a CB2-to-CB1 dose-response ratio of 19.
In vivo	The results provide in vivo evidence for CB1 receptor-mediated increase on endogenous glutamate levels in the cerebral cortex. The Cannabinoid Receptor agonist WIN 55,212-2, at low mg/kg doses, enhances dialysate glutamate levels in the prefrontal cortex of the awake rat.
References	[1] https://pubmed.ncbi.nlm.nih.gov/11459762/ [2] https://pubmed.ncbi.nlm.nih.gov/7565624/

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