Name: Cholesterol
Brand: Selleck Chemicals
SKU: S4154
Rating: 5 (11 reviews)

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Quality Control

Batch: Purity: 99.6%

99.6

Related Targets	Adrenergic Receptor GPR Androgen Receptor Glucocorticoid Receptor ACE RAAS Progesterone Receptor Opioid Receptor PGES THR
Other Estrogen/progestogen Receptor Inhibitors	Elacestrant (RAD1901) Dihydrochloride Vepdegestrant (ARV-471) MPP dihydrochloride Kaempferol G15 Endoxifen HCl Licochalcone A Chrysin Pregnenolone AZD9496

Chemical Information, Storage & Stability

Molecular Weight	386.65	Formula	C₂₇H₄₆O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	57-88-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Cholesterin			Smiles	CC(C)CCCC(C)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)O)C)C

Solubility

In vitro
Batch:

Ethanol : 6.25 mg/mL

DMSO : 0.03 mg/mL (0.07 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Clear solution	5%ethyl alcohol 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.700mg/ml (1.81mM)	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clarified ethyl alcohol stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% ethyl alcohol 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.700mg/ml (1.81mM)	Taking the 1 mL working solution as an example, add 50 μL of 14 mg/ml clear ethyl alcohol stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro

Cholesterol serves as a precursor for the biosynthesis of steroid hormones, bile acids, and vitamin D. It modulates membrane fluidity over the range of physiological temperatures. The hydroxyl group on Cholesterol interacts with the polar head groups of the membrane phospholipids and sphingolipids, while the bulky steroid and the hydrocarbon chain are embedded in the membrane, alongside the nonpolar fatty-acid chain of the other lipids. Through the interaction with the phospholipid fatty-acid chains, Cholesterol increases membrane packing, which reduces membrane fluidity. The structure of the tetracyclic ring of Cholesterol contributes to the decreased fluidity of the cell membrane as the molecule is in a trans conformation making all but the side chain of Cholesterol rigid and planar. In this structural role, Cholesterol reduces the permeability of the plasma membrane to neutral solutes, protons, and sodium ions.

In vivo

Cholesterol is recycled. The liver excretes it in a non-esterified form (via bile) into the digestive tract. Typically about 50% of the excreted Cholesterol is reabsorbed by the small bowel back into the bloodstream.

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT01037387	Recruiting	Chronic Obstructive Pulmonary Disease\|Hypercapnic	Hospital Universitario La Paz	December 2025	Phase 4
NCT06159543	Not yet recruiting	Hyperglycemia\|Dyslipidemias\|Insulin Resistance\|Inflammation\|Oxidative Stress	Loma Linda University\|National Mango Board	September 2024	Not Applicable
NCT06405880	Not yet recruiting	Cardiovascular Risk Factors\|Blood Pressure\|Cardiovascular Disease\|Diabetes\|Dyslipidemia	University of Alberta	September 2024	Not Applicable

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Cholesterol Estrogen/progestogen Receptor agonist

Chemical Structure

Molecular Weight: 386.65