Hedgehog/Smoothened Agonists

Cat.No.	Product Name	Information	Product Use Citations
S1146	Cyclopamine (11-Deoxojervine)	Cyclopamine (11-deoxojervine) is a specific Hedgehog (Hh) signalling pathway antagonist of Smoothened (Smo) with an IC50 of 46 nM in TM3Hh12 cells.	Nat Commun, 2025, 16(1):1267 EMBO Mol Med, 2025, 17(2):336-364 Development, 2025, 152(8)dev204324
S2151	Sonidegib (NVP-LDE225, Erismodegib)	Sonidegib is a Smoothened (Smo) antagonist, inhibiting Hedgehog (Hh) signaling with IC50 of 1.3 nM (mouse) and 2.5 nM (human) in cell-free assays, respectively. Phase 3.	Nat Commun, 2025, 16(1):5137 Nat Commun, 2025, 16(1):1267 Method Protocol, 2025, 8(3)59
S7092	SANT-1	SANT-1 directly binds to Smoothened (Smo) receptor with K_d of 1.2 nM and inhibits Smo agonist effects with IC50 of 20 nM.	Nat Commun, 2024, 15(1):9771 Stem Cell Reports, 2024, 19(7):973-992 Front Immunol, 2023, 14:1258074
S7138	BMS-833923	BMS-833923 (XL139) is an orally bioavailable Smoothened antagonist. Phase 2.	Nat Commun, 2025, 16(1):4983 Sci Rep, 2022, 12(1):7 Mol Cancer Res, 2021, molcanres.MCR-21-0257-A.2021
S2777	PF-5274857	PF-5274857 is a potent and selective Smoothened (Smo) antagonist, inhibits Hedgehog (Hh) signalling with IC50 and Ki of 5.8 nM and 4.6 nM, respectively, and this compound can penetrate the blood–brain barrier.	Sci Rep, 2022, 12(1):7 Biol Reprod, 2021, ioab111 Carcinogenesis, 2020, 41(7):993-1004
E0787	ALLO-2	ALLO-2 is a potent antagonist of both wild-type mouse Smo and drug-resistant D473H mutant human Smo that inhibits Smo agonist inhibited Gli-Luc activity in TM3-Gli-Luc cells with IC50 of 6 nM.
S1182^New	GANT58(NSC75503)	GANT 58 (NSC 75503) is a potent antagonist of GLI-mediated transcription with an IC50 of ≈5 μM. It can be used for research into Ewing Sarcoma and several forms of solid cancer.
E0480	MRT-81	MRT-81 is a potent antagonist of human and rodent smoothened (Smo) receptors, with an IC50 value of 41 nM in the Shh-light2 cells, exerting potent hedgehog (Hh) signalling pathway inhibiting activity.